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Biological Sciences and Biotechnology
Mecca for cutting-edge convergence studies to investigate essentials of vital human phenomenon

Biological Sciences, based on the understanding of each and every biological phenomenon in living organisms, is the cutting-edge scholarship which will constitute of the fundamentals in the 21st century knowledge-based society. The results of scholarship are being utilized for the enhancement of human welfare. Since the turn of the century, the College of Biological Sciences and Biotechnology has been making continuous efforts to reform learning, teaching and research environment by actively participating into the New University Regional Innovation (NURI) Project and the Brain Korea 21 (BK 21) Project. As the government designates the Chungcheon region as one of “National 5+2 Supra Economic Region” in which bio/pharmaceutical and medical industry will be specialized, the college is preparing for a new initiative, the establishment of the training center for bio/pharmaceutical and medical professionals to meet the needs of industry.

Tel+82-42-821-5572 Homepagehttp://sbb.cnu.ac.kr/bk21plus/eng/
Biological Science

“Biological Science (BS)“ is undergraduate program for the education of necessary academic sectors in examining all the levels from the cell, the basic unit of a living creature, to an individual organism, population, and community. The course works of BS contain diverse subjects such as molecular biology, genetics, physiology, ecology, biodiversity and their evolution, and aims to cultivate competent people with a basic knowledge of bioscience and a high adaptive ability through various experiments, fieldworks, and actual practices as well as theoretical lectures. After completing these courses, students can enter relevant companies, or can become professors, researchers, and doctors by entering graduate school or medical school. Students who wish to become middle school teachers may choose the teacher training course.

[Boo, Sung Min]
  • Title : Professor
  • Major : Algal biodiversity and evolution
  • Phone : +82-42-821-6555
  • E-mail : smboo@cnu.ac.kr
  • Room : Building N11 - 407
  • Homepage : www.myalgae.org / http://kmpc.kr
  • Algal diversity and evolution : Our laboratory is actively involved in discovering and analyzing patterns of morphological and molecular evolution in algae and assessing their biogeographic patterns throughout different regions using mitochondrial and plastids genes. Furthermore,hylogenetic relationships of high-level taxa can also produce unexpected finding such as the description of the new order Ishigales and the new genus Gelidiophycus. In this way,we can get a more comprehensive picture of these phylogenetically diverse organisms.
  • Biodiversity and phylogeography of marine algae in korea : The climate change process might have changed the algal flora in the Korean coastal waters,therefore,in our research is also included a long term monitoring of algal diversity to implement a baseline data set for the future and to understand the dynamics of seaweed diversity in Korea. Molecular tool can also be used in this topic. Genetic diversity and generic structure are fundamental characteristics of population that provide insight into the processes affecting the current distribution of species,as well as being important to the growing concern about the preservation of marine biodiversity. We have had Korean Marine Plant Collection Project granted by the Ministry of Oceans and Fisheries.
[Cheol-Hee Kim]
  • Title : Professor
  • Major : Developmental Genetics
  • Phone : +82-42-821-5494
  • E-mail : zebrakim@cnu.ac.kr
  • Room : Building N11-513
  • Fishing novel genes for social behaviorm : The zebrafish is a vertebrate model system suitable for fishing novel genes in post-genome era. About 20 years after my experience in the difficulty of positional cloning,insertional mutagenesis,and after hundreds of anti-sense morpholinos,1 became excited with the prospect of new technology of targeted knockout (KO) based on ZFN,TALEN and now Cas9. We have organized a consortium called "ZEN" (Zebrafish & Engineered Nucleases) in Asia-Oceania region,and executed a pilot study with important novel genes requested from various laboratories. After the first round of injections,we had identified stable mutations in F1 embryos at a very high efficiency. So far,we generated stable knockouts in more than 100 genes,with additional genes currently under screening. We have several surprising results of the analysis of our KO mutant lines,each having phenotype with anxiety, aggression, epilepsy, or autism-like behavior,respectively. Among these,a novel chemokine-like family member,samdori-2 (sam2),is specifically expressed by neurons in the dorsal habenula (Hb) of zebrafish. The Hb is a structure in the epithalamus highly conserved during vertebrate evolution,and mediates behavioral responses to stress,anxiety,and fear. Dysfunction of Hb is associated with depression,post-traumatic stress disorder,and schizophrenia in humans. Targeted knockout (KO) of the sam2 gene did not affect normal development of neural circuits including Hb to interpeduncular nucleus (1PN) pathways. However,sam2 KO fish exhibited strikingly elevated anxiety-like behaviors in the novel tank and scototaxis tests and showed increased social cohesion. With the power of this new genome editing KO technology,we will have a turning point in the biomedical research,such as "Designer Genomics & Bioinnovation".
  • Selected Publications
  • - ZC4H2, an XLID gene, is Required for the Generation of CNS Interneurons. Submitted.
  • - Lee MS et al., IFT46 plays an essential role in cilia development. Dev Biol. 2015
  • - Jang MA et al., Mutations in DDX58, which Encodes RIG-I, Cause Atypical Singleton-Merten Syndrome. Am J Hum Genet. 2015
  • - Kim M et al., The MST1/2-SAV1 complex of the Hippo pathway promotes ciliogenesis. Nat Commun. 2014
  • - So JH et al., FIH-1, a Novel Interactor of Mindbomb, Functions as an Essential Anti-Angiogenic Factor during Zebrafish Vascular Development. PLoS One. 2014
  • - Sung YH et al.,Highly efficient gene knockout in mice and zebrafish with RNA guided endonucleases. Genome Res. 2013
  • - Joo K et al.,CCDC41 is required for ciliary vesicle docking to the mother centriole. Proc Natl Acad Sci U S A. 2013
  • - Ahn D et al.,Evolution of the tbx6/16 subfamily genes in vertebrates: insights from zebrafish. Mol Biol Evol. 2012
  • - Song IS et al.,Inhibition of MKK7 JNK by the TOR signaling pathway regulator like protein contributes to resistance of HCC cells to TRAIL induced apoptosis. Gastroenterology. 2012
  • - Kim HG et al.,Translocations disrupting PHF21A in the Potocki Shaffer syndrome region are associated with intellectual disability and craniofacial anomalies. Am J Hum Genet. 2012
[Eun-hee Kim]
  • Title : Professor
  • Major : Cell Signaling & Tumor Biology
  • Phone : +82-42-821-5495
  • E-mail : eunhee@cnu.ac.kr
  • Room : Building N11-412
  • Programmed cell death (PCD) is an essential regulatory mechanism during an organism’s life cycle. Therefore, PCD is considered as for the most part diseases including neurodegenerative disease, cancer and blindness disease are involved in impairment of it. Recovery of PCD to normal condition can protect various neurons from neurodegeneration and sensitize tumor cells to anti-cancer drug treatment. Therefore, our laboratory aims to find novel PCD regulatory genes which is to be candidate for new drugs target and understand their molecular mechanism.
  • Selected Publications
  • Our lab is interested in - Understanding molecular mechanisms of cell death and pathogenesis mechanisms
  • Current research involves in - Identification and validation of novel death proteins as drug targets - Screening and optimization of small molecules targeting death proteins - Development of disease animal models
[Hankuil Yi]
  • Title : Assistant professor
  • Major : Plant molecular genetics & biochemistry
  • Phone : +82-42-821-6410
  • E-mail : hankuil.yi@cnu.ac.kr
  • Structure/function relationship of plant disease resistance gene
  • In plants, pathogen infection is recognized by disease Resistance (R) proteins, which results in the activation of appropriate defense responses. The expression of R genes is tightly regulated at the multiple levels to avoid deleterious effects on growth and is affected by various environmental conditions. Using disease resistance proteins with nucleotide-binding domain and leucine rich repeat, I am interested in identifying protein regions that play an important role in the activity of R proteins.
  • Substrate specificity of biochemical enzymes in plants
  • Based on functional information obtained from a model plant Arabidopsis, I am interested in identifying key amino acid residues, which are responsible for similar yet distinct biochemical activities and substrate specificity of these enzymes.
[Heon Man Lim]
  • Title : Professor
  • Major : Molecular Biology
  • Phone : +82-42-821-6276
  • E-mail : hmlim@cnu.ac.kr
  • Research Interests
  • A newly found transcription pausing at the beginning of the gal operon
  • Transcription pausing from 238A to 526A was observed for the first time in the galE gene. It was named as TFC pausing (The first cistron pausing). It was composed of 3 clusters: PAUSE I (238A to 286G),PAUSE II (320C to 370A) and PAUSE III (410T to 526A). TFC pausing is DNA sequence dependent and not affected by GreA or GreB. NusA and NusG are involved in regulation of TFC pausing.
  • Molecular mechanisms on differential expression of cistrons in the galactose operon of Escherichia coli
  • In vivo studies were performed to investigate the mechanism of mRNA concentration gradient formation in galactose operon. Using quantitative real-time RT- PCR,it was observed that throughout the growth phase,there were two types of mRNA concentration gradient (mCONGRAD) exist in galactose operon.
  • The CnuK9E H-NS complex antagonizes
  • DNA binding of DicA and leads to temperature-dependent filamentous growth in E. coli Cnu (an OriC-binding nucleoid protein) associates with H-NS. A variant of Cnu was identified as a key factor for filamentous growth of a wild-type Escherichia coli strain at 37"C. The temperature-dependent filamentous growth of E. coli bearing CnuK9E could be reversed by either lowering the temperature to 25"C or lowering the CnuK9E concentration in the cell. Gene expression analysis suggested that downregulation of dicA by CnuK9E causes a burst of dicB transcription,which,in turn,elicits filamentous growth.
  • Selected Publication
  • - Xun Wang,a Sang Chun Ji,a Sang Hoon Yun,a Heung Jin Jeon,a Si Wouk Kim,b Heon M. Lima Expression of Each Cistron in the gal Operon Can Be Regulated by Transcription Termination and Generation of a galK-Specific mRNA, mK2. J. Bacteriol. 2014, 196(14): 2598
  • - Yun,S. H.,Ji,S. C.,Jeon,H. J.,Wang,X.,Kim,S. W.,Bak,G.,Lee,Y. & Lim,H. M. 2012. The CnuK9E H-NS complex antagonizes DNA binding of DicA and leads to temperature-dependent filamentous growth in E.coli. PLos One,(9):e45236
  • - Ji,S. C.,Wang,X.,Yun,S. H.,Jeon,H. J.,Lee H. J.,& Lim,H. M. 2011. In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. PLoS One,6(3):e17646
  • - Lee,H. J.,Jeon,H. J.,Ji,S. C.,Yun,S. H. & Lim,H. M. 2008. Establishment of an mRNA gradient depends on the promoter: an investigation of polarity in gene expression. J Mol Biol 378,318-27.
[Hyunju Ro]
  • Title : Associate professor
  • Major : Cellular Differentiation
  • Phone : +82-42-821-5496
  • E-mail : rohyunju@cnu.ac.kr
  • Research interests: Proteostasis during vertebrate development
  • Ubiquitin-dependent protein modification process is critical not only for the target proteins destruction but also for the cellular signaling, protein trafficking, endocytosis etc. Several types of human diseases are implicated in the defective protein ubiquitylation, reflecting the importance of the protein ubiquitylation in the cellular physiology. To data, the ubiquitin-dependent protein modification mechanism has been extensively studied in in vitro and homogenous cell culture systems. However, the in vivo functions of individual E3 ubiquitin ligases during vertebrate embryonic development still remain as enigma. The early vertebrate embryonic development is initiated through the crosstalk and collaboration of Wnt, TGFb and FGF signaling. The combined action of the signaling pathways initially determines dorso-ventral body axis followed by anterior-posterior axis and left-right body asymmetry. For instance, aberrant regulation of RAS signaling pathway, which is largely induced by FGF/EGF/VEGF stimuli, is the one of the main causative factors of the cardio-facio-cutaneous syndrome and malignant melanoma. Since these signaling pathways play important roles for the embryonic axis formation as well as diverse post-embryonic pathogenesis, it is important to study the molecular mechanisms using early developing embryos with suitable model system to gain insights into genetic disorders elicited by aberrant modulation of the signaling pathways. The zebrafish model organism is suitable for the study of vertebrate embryonic development and cellular signaling mechanism. Using zebrafish as an animal model system, we’ve studied several unique E3 ubiquitin ligases showing dynamic spatio-temporal expression pattern during early embryonic development. Currently we’ve been tested the possible in vivo modalities of a specific E3 and RAS signaling regarding to the Noonan syndrome. To date, few E3 ubiquitin ligases have been reported to play a role for the early vertebrate embryonic development. Given that there are 500 different E3 ubiquitin ligases and that approximately 30% of genes are estimated to be critical for the early embryonic development, at least 100 different E3 ubiquitin ligases are predicted to be involved in tissue specification and differentiation during prenatal stage. So far the molecular mechanisms underlying the ubiquitin dependent protein modification processes have been extensively studied in the fields of cell biology, molecular biology and biochemistry. However, how specific modification of proteins with ubiqutin affects embryonic development remains largely unknown. Thus, understanding of early embryonic development is important to gain insights into the in vivo function of genes which are related to postnatal human diseases. Taken together, it opens up promising analytical prospects in the field of biology to study the molecular functions of E3 ubiquitin ligases during early embryonic development.
[Kee-Jeong Ahn]
  • Title : Professor
  • Major : Systematic Zoology (Beetle Diversity)
  • Phone : +82-42-821-5492
  • E-mail : kjahn@cnu.ac.kr
  • Homepagecafius.wix.com/korean-beetles
  • Research Interests
  • - Systematics, Phylogeny, Biogeography, and Evolution of the coastal Staphylinidae (Insecta: Coleoptera)
  • - Phylogenetic Relationships of the Aleocharinae (Coleoptera: Staphylinidae)
  • - Revision of the East Asian Aleocharinae (Coleoptera: Staphylinidae)
  • - Systematics of the Staphylinoidea (Insecta: Coleoptera) in Korea
  • - Beetle Diversity in Korea
  • Selected Publication
  • - Song, J.-H. and K.-J. Ahn. 2014. Species delimitation in the Aleochara fucicola species complex (Coleoptera: Staphylinidae: Aleocharinae) and its phylogenetic relationships. Zoologica Scripta 43 (6) : 629-640.
  • - Park, S.-J., R. A. B. Leschen and K.-J. Ahn. 2014. Phylogeny of the Agathidiini Westood (Coleoptera: Leiodidae) and implications for classification and contractile morphology. Systematic Entomology 39: 36-48.
  • - Jeon, M.-J., J.-H. Song and K.-J. Ahn. 2012. Molecular phylogeny of the marine littoral genus Cafius (Coleoptera: Staphylinidae: Staphylininae) and implications for classification. Zoologica Scripta 41: 150-159.
  • - Frank, J. H. and K.-J. Ahn. 2011. Coastal Staphylinidae (Coleoptera): A worldwide checklist, biogeography and natural history. Zookeys 107: 1-98.
  • - Ahn, K.-J., M.-J. Jeon and M. A. Branham. 2010. Phylogeny, biogeography and the stepwise evolutionary colonization of intertidal habitat in the Liparocephalini based on morphological and molecular characters (Coleoptera: Staphylinidae: Aleocharinae). Cladistics 26: 344-358.
[Kwang-Guk An]
  • Title : Professor
  • Major : Environmental Ecology
  • Phone : +82-42-821-6408
  • E-mail : kgan@cnu.ac.kr
  • Stream ecosystem health assessments using fish bioindicators and biomarkers
  • Multi-metric fish models, based on fish bioindicators (community data), are studied in the lotic ecosystems and lentic ecosystems and the approach is based on the Index of Biological Integrity (IBI). These model values are compared with chemical water qaulity parameters (N, P, BOD, COD etc) and habitat conditions of Quantitative Habitat Evaluation Index (QHEI). Thus, stream health conditions, based on fish bioindicators, are diagnosed by the criteria of impairment degree. In addition, for the health assessments, we use low-level biomarkers such as a comet assay for DNA damage analysis and physiological assays of EROD activity and TOSC analysis. The present bioassessment methodology may be used as a key tool to set up specific goals for stream restoration plans as well as a biological measure diagnosing current stream conditions.
  • Nutrient dynamics and eutrophication processes
  • Eutrophication in aquatic ecosystem is also one of our major research topics. Various limnological/hydrological parameters are measured from various man-made lakes,and chemical water quality is evaluated on the basis of the analysis. Also,in situ Nutrient Stimulation Bioassays (NSBs) are conducted for determining nutrient limitation on phytoplankton production in various lentic ecosystems. Such experiments demonstrate how the eutrophication is processed in the ecosystem and which factor is a key factor regulating the algal growth.
  • Empirical modeling of nutrients and trophic dynamics of nutrients-to-fish
  • We develop empirical models of chlorophyll-a (CHL) - total phosphorus (TP) in different Korean reservoirs and study how Asian monsoon influences relationships between chlorophyll-a (CHL) and total phosphorus (TP). Also,we test some effects of nitrogen or other nutrient additions on algal growth using the cubitainers. These relations are controlled by intensity of Asian monsoon or upper-dam discharges, so we analyze seasonal and inter-annual patterns between the nutrients and CHL. And then we study how the nutrients are associated with higher trophic organisms of fish, so bottom-up and top-down hypotheses are tested in in situ mesocosms as well as in the laboratory.
  • Ecological Impact assessments of dam/weir’s constructions on fish migration
  • The dams and large-size weirs are the major physical barriors in the stream and river ecosystem. The dams and weirs are everywhere in Korea, so the ecological impacts are expected in the lotic ecosystems, so we test how the artificial facilities influence on the short-distance movements and long-range migrations of fishes in the weirs and fishways (fish passage) in the four major rivers.
  • Ecological risk assessments
  • Also,we conduct ecological risk assessments using ecological target species (fish) or invertebrate (daphnia, bryozoa etc.) within the laboratory or in situ to know whether specific chemicals influence the biota in the waterbodies or not.
[Kwan-Hee You]
  • Title : Professor
  • Major : Developmental Biology
  • Phone : +82-42-821-5498
  • E-mail : khyou@cnu.ac.kr
  • Room : Building N11-511
  • Clusterin is involved in the variety of physiological functions such as cell aggregation,lipid transportation,perm maturation,inhibition of complement activation,nhancing transport,and stabilization of peptide hormones. It is also involved in neurodegenerative diseases,the differentiation and proliferation of epidermal and muscle cells,morphogenic processes,nd plasma membrane reorganization during apoptosis. These multi-functions suggest that the potential role of clusterin is a biological detergent or a chaperone of secretory protein. Clusterin has been also implicated to play a role in tumorogenesis,cardiovascular diseases,nd neurological disorders. Clusterin is considered as a secreted protein. However,we have isolated and characterized the isoform of clusterin and showed that this isoform is translocated into the nucleus to perform its function when TGF-beta was present. The detailed analysis of clusterin are as in the following:
  • - Study of intracellular trafficking of clusterin. Molecular study of clusterin expression and apoptosis in TGF-beta treated cells.
  • - Study of intracellular trafficking of clusterin. Molecular study of clusterin expression and apoptosis in TGF-beta treated cells.
  • - Study of the biochemical interaction between clusterin and chaperones.
  • - Study of the folding and secretion of clusterin at the celluar level.
  • - Study of the genetic interaction between clusterin and PLZF using transgenic flies
  • - Study of the genetic interactions between clusterin and TGF-beta signaling during apoptosis in the Drosophila model system
[Man-Ho Oh]
  • Title : Associate Professor
  • Major : Receptor Kinases Signaling in Plants
  • Phone : +82-42-821-5497
  • E-mail : manhooh@cnu.ac.kr
  • Plants response to developmental and environmental signals in part through membrane-localized receptor kinases that interact with other proteins to initiate a cascade of biochemical events resulting in altered cellular function. Key to understanding LRR-RLKs action in specific signaling pathways is the identification of both membrane-bound and soluble protein partners. However, the functions and interacting protein networks of the vast majority of this large family of signal transduction molecules remain unknown. In plants, binding of the ligand to the receptor’s extracellular domain induces interaction with a co-receptor kinase. Juxtaposition of the two cytoplasmic protein kinase domains then results in their activation via auto- and/or trans-phosphorylation, and a signal transduction cascade that alters gene expression in the nucleus. We are broadly interested in the mechanisms that regulate the formation and activity of the receptor kinase signaling complex, including post-translational modifications such as phosphorylation. Therefore, we are focusing on the diversification and amplification of signaling pathways using interdisciplinary approaches from plant genetics, biochemistry, mass spectrometry and proteomics, an extensive network of LRR-RLK interacting proteins and signal transduction including brassinosteroid hormone.
[Myungchull Rhee]
  • Title : Professor
  • Major : NeuroDevelopmental Genetics
  • Phone : +82-42-821-6278
  • E-mail : mrhee@cnu.ac.kr
  • We are currently addressing two questions in mammalian cells and zebrafish animal model system; (1) How ubiquitin ligases exert their control over the brain patterning and neuronal differentiation (2) What are the essential regulator for the differentiation of dopaminergic and seratonergic neurons from the progenitor and precursor cells. In search of elements and networks associated with the first question we have identified CNS-specific ubiquitin ligases and their target proteins. For thorough screening essential regulators for the second question we are currently conducting dynamic studies using generation of transgenic animals, deep sequencing, transcriptomic analysis,bioinformatics,and functional genomics.
  • Ubiquitin (Ub) Ligases and Targets for the Brain Patterning
  • We have identified brain-specific Ub ligases,their targets and regulators. The work has generated networks which are profoundly associated with brain development as well as neuronal differentiation particularly in the central nervous system. We are recently extending the list of the brain-specific E3 ligases and their upstream and downstream partners,which shall give rise to better comprehensive networks for the vertebrate brain development and differentiation.
  • Transcriptomes, Bioinformatics, and Functional Genomics for the Molecular Networks of Dopaminergic and Seratonergic Neuronal Differentiation
  • Although stem cell technologies have been leaping for the past decade,detailed networks governing specification of neuronal stem cells as well as terminally differentiation neuronal cells remain to be investigated. We are thus attempting to search for molecular regulators involved in the differentiation using current tools,uch as single cell analysis with deep sequencing,generation of networks with bioinformatics,and functional genomics with engineered nucleases and caspase/ssRNA system.
[Seong-Deog Kim]
  • Title : Professor
  • Major : Plant Ecolgy
  • Phone : +82-42-821-6556
  • E-mail : sdkim@cnu.ac.kr
  • CO2 sink assessments in forest and agro-ecosystems
  • Long-term monitoring and accurate measuring the net ecosystem production (NEP) in forest ecosystems are important to understand the carbon balance under current and future global warming environments. Our study on carbon sink assessments is based on the biometric method in the forest and the agro- ecosystems through out the tropical,temperated and subarctic region. We have CO2 carried out the measurements of biomass,net primary productivity (NPP),soil CO2 efflux,stem respiration and net ecosystem productivity (NEP) in Korea,Thailand and Alaska since 2003.
  • Classification of vegetation (phytosociological study)
  • Plant community has studied from two standing points.One is focusing on its classification and the other on its dynamics. Our phytosociological studies aim to recognize vegetational units through finding the diagnomic species in their full floristic composition of communities,and to analyze its correlation with environments. We have extensively investigated all vegetation types in Korea as well as some vegetation types in China and Japan.
  • Dynamice of the forest(Regeneration process of the forest)
  • Forest communities are mosaics of patches,which develop as the result of natural disturbances. And regeneration occurs in gaps created by the death of canopy trees. In general,the mosaic theory is accepted as a valuable concept for understanding dynamics of forest communities. We clarified the regeneration process on Korean beech forest,Mongolian oak forest,and red pine forest.
[Woongghi Shin ]
  • Title : Professor
  • Major : Protist Evolution and Phylogenetics
  • Phone : +82-42-821-6409
  • E-mail : shinw@cnu.ac.kr
  • My research concentrates on biodiversity and evolutionary history of photosynthetic protists based on ultrastructure and molecular data
  • Ultrastructure
  • Protist is a large and diverse group of eukaryotic organisms,which belong to the kingdom Protista. Some of them are obviously phototrophs,while others are either osmotrophs,mixotrophs or phagotrophs. Aim of my study is to clarify evolutionary trend of selected ultrastructural features which are 1) basal body complex,2) feeding apparatus,and 3) microtubular cytoskeleton.
  • Molecular phylogeny
  • Recent advances in systematics,particularly the use of molecular data analyzed with objective methods,have made it practical to reconstruct the history or evolution of protists. Information on how photosynthetic protists are related to each other can be used to infer how protists evolved. I have sequenced many genes (especially,nuclear- and plastid-encoded),which are given us a lot of information regarding phylogeny of photosynthetic protists. Now my lab is trying to understand phylogenetic relationships among taxa and evolution of protists.
  • Selected Publication
  • - Choi,B.,Son,M.,Kim,J. 1.,Shin,W. 2013. Taxonomy and phylogeny of the genus Cryptomonas (Cryptophyceae,Cryptophyta) from Korea. Algae 28: 307-330.
  • - Nam,S.W.,Go,D.,Son,M.,Shin,W. 2013. Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae,Cryptophyta). Algae28: 331-341.
  • - Kim,J.1.,Shin,W.,Triemer,R.E. 2013. Phylogenetic reappraisal of the genus Monomorphina (Euglenophyceae) based on molecular and morphological data. J. Phycol. 49:82-91.
  • - Kim,J. 1.,Shin,W.,Triemer,R. E. 2013. Cryptic speciation in the genus Cryptoglena (Euglenaceae) revealed by nuclear and plastid SSU and LSU rRNA gene. J. Phycol. 49:92-102.
  • - Jo,B.Y.,Shin,W.,Kim,H.S.,Siver,P. A.,Andersen,R. A. 2013. Phylogeny of the genus Mallomonas (Synurophyceae) and descriptions of five new species on the basis of morphological evidence. Phycologia 52:266-278.
  • - Siver,P.A.,Wolfe,P.A.,Rohlf,F.J.,Shin,W.,Jo,B.Y. 2013. Combining geometric morphometrics,molecular phylogeny,and micropaleontology to assess evolutionary patterns in Mallomonas (Synurophyceae: Heterokontophyta). Geobiology 11:127-138
  • - Nam,S.W.,Shin,W.,Coats,D.W.,Park,J.W.,Yih,W. 2012. Ultrastructure of the oral apparatus of Mesodinium rubrum from Korea. J. Eukaryot. Microbiol. 59:625-636.
  • - Jeong,H.J.,Yoo,Y.D.,Kang,N.S.,Lim,A.S.,Seong,K.A.,Lee,S.Y.,Lee,M.J.,ee,K.H.,Kim,H.S.,Shin,W.,Nam,S.W.,Yih,W.,Lee,K. 2012. Heterotrophic feeding as a newly identified survival strategy of the dinoflagellate Symbiodinium. PNAS. 109:12604-12609.
  • - Kottuparambil,S.,Shin,W.,Brown,M.T.,Han,T. 2012. UV-B affects photosynthesis,ROS production and motility of the freshwater flagellate,uglena agilis Carter. Aquatic Toxicol. 122-123:206-213.
  • - Kim,M.,Nam,S.W.,Shin,W.,Coats,D.W.,Park,M.G. 2012. Dinophysis caudata (Dinophyceae) sequesters and retains plastids from the mixotrophic ciliate prey Mesodinium rubrum. J. Phycol. 48:569-579.
[Yoonkang Hur]
  • Title : Professor
  • Major : Plant Molecular Biology
  • Phone : 82-42-821-6279
  • E-mail : ykhur@cnu.ac.kr
  • Functional genomics study in chinese cabbage
  • Using Brassica rapa oligomer chips (Br300K and Brl35K microarray) and RNA Seq,we have analyzed gene expression profiles and identified genes of interest from B. rapa and B. oleracea. Most genes of interest are genes involved in pollen fertility,freezing tolerance,drought stress,leaf color and high temperature tolerance.
  • Molecular marker development in Brassica
  • To assist molecular breeding of Brassica crops,we have developed gene-specific molecular markers that are related to flowering time,temperature-stress tolerance,and anthocyanin biosynthesis. Target genes for developing molecular markers have been selected by transcriptome analyses,and followed by genomic sequence analysis. We are currently identified many gene-specific PCR-markers and thousands of SNPs and SSRs.
  • Functional study on unknown genes
  • We have identified many genes that would regulate desirable agronomic traits as well as biological phenomena. To elucidate their function,we used transgenic technologies,biochemistry and genetic approaches. We are currently using Arabidopsis,B.napus and B. rapa transgenic plants.
[Youn-il Park]
  • Title : Professor
  • Major : Plant Functional Genomics
  • Phone : +82-42-821-5493
  • E-mail : yipark@cnu.ac.kr
  • Homepagehttp://cnuppl.or.kr
  • Light Sensing and Signaling in Oxygenic Photoautotrophs
  • Photosynthetic organisms optimize their photosynthetic performance in response to ever changing light environments by virtue of a range of photosensory and signaling systems. Sensing light cues are mediated by photoreceptors that consist of apoproteins and chromophores including tetrapyrroles,flavins or p- coumaric acid,vitamin Bl2 and pterins. Among these chromophores,bilin species like biliverdin,phycocyanobilin and phytochromobilin derived from the oxidative degradation of heme are ubiquitous in phytochrome superfamily photoreceptors. Till today,cyanobacterial phytochromes characterized in depth are mostly originated from unicellular freshwater Synechocystis sp. PCC 6803 or nitrogen fixing mutlicelluar filamentous cyanobacteria Nostoc punctiforme ATCC29l33 and Anabaena sp. PCC 7l20. However,light sensing and signaling machinery is barely known in mat forming cyanobacteria from extreme environments such as saline lagoons,thermal springs,or soda lakes. Such habitats featuring high content of carbonates and high levels of pH contain unicellular CRhabdoderma,Euhalothece and Synechococcus) and multicellular filamentous CMicrocoleus,Phormidium and Mastigocladus) cyanobacteria. Recently,we sequenced a dozen genomes of extremophilic cyanobacteria including Microcoleus,Rhabdoderma,Euhalothece that was diverged between 0.67 - l.5 BYA and systematic approaches are undertaken to explore biochemical and biological functions of mostly bilin based photoreceptors primarily aiming for our understanding of ancient photosensory networks and accordingly evolution of the photosensory modules. The second ongoing project is related to the anthocyanins recognized as light screening pigments protecting photosynthetic apparatus from harmful light in addition to their diverse functions. Among many of positive or negative transcription factors,an R2R3-MYB transcription factor PAPl is differentially modulated at the transcriptional and post-transcriptional levels by environmental and biological factors such as light,temperature,sugar and hormones. Currently,everal regulatory factors initially screened by reverse genetics approaches using Arabidopsis paplD gain-of-function mutants are characterized,hoping to fill in some blank leading to transcriptional activation of PAPl expression.
Curriculum
Microbiology & Molecular Biology
Introduction

The educational goal in the Department of Microbiology and Molecular biology at CNU is to meet the strong demands for research scientists and professionals in modern life sciences. The department has nine professors and several adjunct faculty members who are affiliated with research institutes or biotechnology companies. The current enrollment for the undergraduate program is 150 students. The graduate programs currently have 40 students in MS degree program and 30 students in PhD program.

Over the past 10 years, significant progress has been made toward developing excellent education programs and improving research activities and facilities. The curriculum is designed to make students acquainted with the basic disciplines of microbiology and molecular biology. Especially, for undergraduate students, the department offers intensive laboratory courses covering the whole range of microbiology and molecular biology. Research programs focus largely on the basic disciplines of life science at the biochemical, genetic, molecular and cellular level. However, many applied researches are also carried out in cooperation with industrials and governmental agencies. Since the CNU is located in “Taedok Science Town”, the department has developed cooperative research programs with the institutes and biotechnology companies in the Science Town.

Most of undergraduate students and all graduate students in the department are financially supported by scholarships from various funding sources, such as Creative Korea (CK) program, Brain Korea (BK) 21+ program and research grants. This department is one of the top educational and research institutes for life science in Korea.

Career Opportunities

Most of undergraduate students are employed after graduation or go to graduate school, and almost all of graduate students are employed in biotechnology companies or research institutes right after graduation.

Faculty
[Rhee, Young-Ha]
  • Title : Professor
  • Major : Applied & Environmental Microbiology
  • Phone : +82 42-821-6413
  • E-mail : yhrhee@cnu.ac.kr
  • Room : Building N11 - 604
  • Research Interests
    • Biosynthesis and modification of PHAs
      Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by bacteria as intracellular storage materials. We have isolated several microorganisms that have very interesting characteristics regarding PHA biosynthesis, and developed novel PHAs containing various functional groups, including cyclohexyl, alkoxy, and unsaturated groups in the side chains. Chemical modifications of these PHAs to produce materials with improved properties which are suitable for biomedical applications have been also developed. We are currently investigating the potential use of waste materials as the cheap carbon substrates for the production of PHAs by mixed cultures enriched under aerobic dynamic feeding.
    • Biodegradation of PHAs
      The ability of microorganisms to degrade PHAs is dependent on the secretion of specific PHA depolymerases. Our research has been focused on studies of medium-chain-length (MCL) PHA depolymerases that are specific to polymers consisting of 3-hydroxyalkanoates with six or more carbon atoms. We have reported biochemical characteristics of MCL-PHA depolymerases obtained from various bacterial origins. Biodiversity of microorganisms that degrade MCL-PHA and molecular characteristics of MCL-PHA depolymerase genes are currently underway.
    • Selected Publication
      • Kim et al. 2013. Polyester synthesis genes associated with stress resistance are involved in an insect bacterium symbiosis. Proc. Natl. Acad. Sci. USA 110, E2381-E2389.
      • Seo et al. 2012. Non-ionic polysorbate surfactants: Alternative inducers of medium- chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for production of extracellular MCL-PHA depolymerases. Bioresour. Technol. 121, 47-53.
      • Chung et al. 2012. Overexpression of the (R)-specific enoyl-CoA hydratase gene from Pseudomonas chlororaphis HS21 in Pseudomonas strains for the biosynthesis of polyhydroxyalkanoates of altered monomer composition. Biosci. Biotechnol. Biochem. 76, 613-616.
      • Chung et al. 2012. Biocompatibility and antimicrobial activity of poly(3-hydroxy- octanoate) grafted with vinylimidazole. Int. J. Biol. Macromol. 50, 310-316.
      • Lee et al. 2011. Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid. Bioresour. Technol. 102, 6159-6166.
      • Ni et al. 2010. Biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by volatile aromatic hydrocarbons-degrading Pseudomonas fulva TY16. Bioresour. Technol. 101, 8485-8488.
      • Kim et al. 2010. Imparting durable antimicrobial properties to cotton fabrics using alginate-quaternary ammonium complex nanoparticles. Carbohydrate Polymers 79, 1057-1062.
      • Kim et al. 2008. Graft copolymerization of glycerol 1,3-diglycerolate diacrylate onto poly(3-hydroxyoctanoate) to improve physical properties and biocompatibility. Int. J. Biol. Macromol. 43, 307-313.
      • Kim et al. 2007. Biosynthesis, modification, and biodegradation of bacterial medium-chain-length polyhydroxyalkanoates. J. Microbiol. 45, 87-97.
[Maeng, Pil-Jae]
  • Title : Professor
  • Major : Molecular Cell Biology
  • Phone : +82 42-821-6415
  • E-mail : pjmaeng@cnu.ac.kr
  • Room : Building N11 - 504
  • Research Interests

    Stress resistance- and metabolic disease-related functions of the enzymes involved in TCA cycle and glutamate metabolism in yeast As in multicellular organisms, the unicellular eukaryotic microorganism Saccharomyces cerevisiae is subjected to stress-induced programmed cell death (PCD). We are studying on the mechanism of the stress-induced PCD in the yeast cells lacking the enzymes involved in TCA cycle or glutamate metabolism, e.g., citrate synthase and glutamate dehydrogenase. We also concentrate our efforts on developing the yeast model of metabolic diseases caused by malfunctioning of the TCA cycle or glutamate metabolism.

    Genetic network of development and secondary metabolism in filamentous fungi The filamentous fungus A. nidulans has two distinct types of reproductive event, asexual and sexual cycles, which offer many common developmental themes. To understand the molecular genetic and biochemical mechanism governing the whole process of life cycle, we have performed global gene expression profiling and cluster analysis of A. nidulans during vegetative growth and sexual/asexual differentiation. On the basis of the transcriptome profiling, we are performing functional characterization of several novel putative transcription factors expected to be involved in sexual/asexual differentiation and secondary metabolite production.

    Development and functional characterization of the novel biomarkers for qRT-PCR-based breast cancer diagnosis Breast cancer is currently diagnosed on the basis of several histopathological features including tumor size, grade and lymph node status. In addition, hormone receptors (ER, PR) and HER2 expression in tumors are used to classify and monitor its severity and progression. Despite these common biomarker analyses and the information they provide regarding the molecular features and stage of breast tumors, there remains an unmet need for novel diagnostic and prognostic biomarkers for breast cancer with improved sensitivity and specificity. We screened 25 candidates for potential breast cancer biomarkers, and developed optimal combinations of several novel biomarkers expected to be useful for qRT-PCR-based diagnosis of breast cancer. We are also performing functional characterization of the novel biomarkers.

  • Selected Publication
    • Park, H.S., Y.M. Yu, M.K. Lee, P.J. Maeng, S.C. Kim, J.H. Yu. 2015. Velvet-mediated repression of β-glucan synthesis in Aspergillus nidulans spores. Sci Rep 5, 10199.
    • Park, D.S., Y.M. Yu, Y.J. Kim, P.J. Maeng. 2015. Negative regulation of the vacuole-mediated resistance to K+ stress by a novel C2H2 zinc finger transcription factor encoded by aslA in Aspergillus nidulans. J Microbiol 53, 100-110.
    • Lee, Y.J., K.J. Kim, H.Y. Kang, H.R. Kim, and P.J. Maeng. 2012. Involvement of GDH3-encoded NADP+-dependent glutamate dehydrogenase in yeast cell resistance to stress-induced apoptosis in stationary-phase cells. J Biol Chem 287, 44221-44233.
    • Kim, L., K.L. Hoe, Y.M. Yu, J. Yeon, and P.J. Maeng. 2012. The fission yeast GATA factor, Gaf1, modulates sexual development via direct down-regulation of ste11+ expression in response to nitrogen starvation. PLoS One 7, e42409.
    • Lee, Y.J., J.W. Jang, K.J. Kim, and P.J. Maeng. 2011. TCA cycle-independent acetate metabolism via the glyoxylate cycle in Saccharomyces cerevisiae. Yeast 28, 153-166.
    • Ko, Y.J., Y.M. Yu, G.B. Kim, G.W. Lee, and P.J. Maeng, S. Kim, A. Floyd, J. Heitman, and Y.S. Bahn. 2009. Remodeling of global transcription patterns of Cryptococcus neoformans genes mediated by the stress-activated HOG signaling pathways. Eukaryot Cell 8, 1197-1217.
    • Lee, Y.J., K.L. Hoe, and P.J. Maeng. 2007. Yeast cells lacking the CIT1-encoded mitochondrial citrate synthase are hypersusceptible to heat- or aging-induced apoptosis. Mol Biol Cell 18, 3556-3567.
    [Kim, Jinmi]
    • Title : Professor
    • Major : Microbial Genetics
    • Phone : +82 42-821-6416
    • E-mail : jmkim@cnu.ac.kr
    • Room : Building N11 – 506
    • Research Interests
      • mRNA modulating factors and posttranscriptional regulation Control of mRNA translation, stability, and subcellular location is a key aspect of gene expression regulation in eukaryotic cells. A variety of mRNA modulating systems contributes to this gene expression regulation. We are interested in unveiling the regulatory roles of RNA helicases (Dhh1, Rok1), mRNA decapping activators (Dhh1, Pat1, Lsm1), or translation initiation factors (Caf20, Eap1). Specific mRNAs, including Ste12 transcription factor or Cln1 cell-cycle regulator, which are preferentially regulated at the translation level during yeast mating or filamentous growth pathway are targets of our investigation.
      • mRNA granule, P-bodies and stress granules, and gene expression in pathogenic yeast Candida albicans Under various stress conditions, mRNAs assemble into non-translating mRNPs (messenger ribonucleoproteins), which can concentrate in microscopically visible foci known as processing bodies (P-bodies) or stress granules. mRNAs in these granules can either be degraded or stored for later translation. The virulence conditions responsible for C. albicans pathogenicity are very much relevant to P-body formation. The core components of P-bodies, Dcp2, Dhh1, Kem1/Xrn1, and Edc3, were identified in C. albicans and their localizations with respect to P-bodies were demonstrated. Deletion of P-body scaffolding protein Edc3 attenuated stress-induced responses, including cell death and ROS accumulation. Our current interest is to investigate the Edc3-dependent gene expression regulation.
      • uORF-mediated translational regulation The translational efficiency of eukaryotic mRNAs is often influenced by structural features of the 5’untranslated region (UTR), which include length, secondary structure, and the presence of upstream open reading frames (uORFs). We identified two upstream open reading frames (uORFs) within the ROK1 5’untranslated region, which inhibited Rok1 translation. Rok1 protein levels oscillated during the cell cycle, declining at G1/S and increasing at G2. The uAAG1 mutation caused a constitutive level of Rok1 proteins throughout the cell cycle, resulting in significant delays in mitotic bud emergence and recovery from pheromone arrest. Our study reveals that the Rok1 protein level is regulated by uORFs, which is critical in cell cycle progression. Our current study is focused on the roles of Psk2, Tub4, and Skp1proteins that interact with the 5’UTR of ROK1 mRNA.
    • Selected Publication
      • Jung, J.-H. and J. Kim. 2011. Accumulation of P-bodies in Candida albicans under different stress and filamentous growth conditions. Fungal Genet. Biol. 48, 1116-1123.
      • Jeon, S. and J. Kim. 2010. Upstream open reading frames regulate the cell cycledependent expression of the RNA helicase Rok1 in Saccharomyces cerevisiae. FEBS Letter 584, 4593-4598.
      • Kim, S.-Y. and J. Kim. 2010. Roles of dihydrolipoamide dehydrogenase Lpd1 in Candida albicans. Fungal Genet. Biol. 47, 782-788.
    [Park, Hee-Moon]
    • Title : Professor
    • Major : Cellular Differentiation
    • Phone : +82 42-821-6417
    • E-mail : hmpark@cnu.ac.kr
    • Room : Building N11 - 508
    • Research Interests

      Regulatory circuits of the dual-specificity LAMMER kinase for eukaryotic cell differentiation We have found that abolition of a dual-specificity LAMMER kinase, Lkh1p, exerts profound effects on differentiation of the fission yeast, Schizosaccharomyces pombe, including oxidative stress response, cell division cycle, mating, and so on. Our recent study also revealed that the Lkh1p modulates various cellular events by regulating gene-expression and activity of key regulatory proteins for eukaryotic cell differentiation. At present, we are trying to identify novel regulatory circuits mediated by the LAMMER protein kinase family in yeasts, filamentous fungi, and human.

      Functional analysis of the Aspergillus nidulans developmental genes With the aids of various screening technologies such as proteome, DEG (differentially expressed gene) and real-time PCR analysis, we have identified developmental stage-specific genes modulated by the well-known regulatory factors, VeA, NsdD, and etc. In order to characterize the function of genes and the regulation mode of gene-expressions, we are performing the gene disruption, suppression test, protein-protein interaction, and morphogenetic analysis.

      Biosynthesis and assembly of the fungal cell wall components Fungal cell walls are known as a model for the morphogenetic study and a target for antifungal drug development. Using the cell wall defective mutants of the yeast Saccharomyces cerevisiae and the filamentous fungus Aspergillus nidulans, functional link between intracellular vesicular transport system and cell wall biogenesis, and roles of cell surface proteins in morphogenesis are under investigating.

    • Selected Publication
      • Park BC et al. 2014. Transcriptional regulation of fksA, a β-1,3-glucan synthase gene, by the APSES protein StuA during Aspergillus nidulans development. Jour. Microbiol. 52, 940-947.
      • Kang EH et al. 2013. LAMMER kinase LkhA plays multiple roles in the vegetative growth and asexual and sexual development of Aspergillus nidulans. PLOS ONE. 8, e58762..
      • Yu EY et al. 2013. Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDKinhibitor Rum1. Biochem Biophys Res Commun. 432, 80-83..
      • Kim KH et al. 2011. Effect of Saccharomyces cerevisiae ret1-1 mutation on glycosylation and localization of the secretome. Molc & Cells. 31, 151-158..
      • Kang WH et al. 2010. The Lammer kinase homolog, Lkh1, regulates Tup transcriptional repressors through phosphorylation in Schizosaccharomyces pombe. Jour. Biol. Chem. 285, 13797-13806..
      • Kim DU, et al. 2010. Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe. Nature Biotechnol. 28, 617-623..
    [Ahn, Jeong-Keun]
    • Title : Professor International Business and Finance
    • Major : Molecular Virology
    • Phone : +82 42-821-6418
    • E-mail : jkahn@cnu.ac.kr
    • Room : Building N11 - 614
    • Research Interests

      The functional analysis of Hepatitis B virus X protein
      Human hepatitis B virus (HBV) is a major pathogen for liver diseases including cirrhosis and hepatocellular carcinoma. Among the HBV proteins, X protein plays critical roles in HBV replication and liver oncogenesis. However, the promiscuous functions of HBx are not elucidated yet. We are trying to validate the various regulatory functions of HBx protein in transcriptional transactivation, signal transduction, and carcinogenesis.

      The regulatory mechanisms of HSV-1 viral proteins
      Herpes simplex virus type 1 (HSV-1) is a neurotropic virus which causes a variety of diseases in human. Among immediate early proteins of HSV-1, ICP27 is essential for viral replication and has multiple functions at transcriptional and post-transcriptional levels. We are dissecting the functional mechanism of ICP27 in the regulation of signal transduction, apoptosis, and viral reactivation. In addition, we also carry the functional analysis of ICP22 and glycoproteins of HSV-1..

      Ribozyme and RNA interference
      Ribozymes are catalytic RNA molecules which bind target RNA sequences and cleave them in a sequence specific manner. We are developing the highly efficient ribozymes which are suppressing the specific viral gene expressions..

    • Selected Publication
      • Kim, H.J., Kim, J.C., Min, J.S., Kim, M.J., Kim, J.A., Kor, M.H., Yoo, H.S., Ahn, J.K., 2011. Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-kappaB signaling in liver cancer cells. Journal of Ethnopharmacology 136, 197-203.
      • Kim, H.J., Kim, S.Y., Kim, J., Lee, H., Choi, M., Kim, J.K., Ahn, J.K., 2008. Hepatitis B virus X protein induces apoptosis by enhancing translocation of Bax to mitochondria. IUBMB Life 60, 473-480.
      • Kim, J.C., Lee, S.Y., Kim, S.Y., Kim, J.K., Kim, H.J., Lee, H.M., Choi, M.S., Min, J.S.,
      • Kim, M.J., Choi, H.S., Ahn, J.K., 2008. HSV-1 ICP27 suppresses NF-kappaB activity by stabilizing IkappaBalpha. FEBS Letters 582, 2371-2376.
      • Park, J., Lee, J.H., La, M., Jang, M.J., Chae, G.W., Kim, S.B., Tak, H., Jung, Y., Byun, B., Ahn, J.K., Joe, C.O., 2007. Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. Journal of Molecular Biology 368, 388-397.
      • Kim, S.Y., Kim, J.K., Kim, H.J., Ahn, J.K., 2005. Hepatitis B virus X protein sensitizes UV-induced apoptosis by transcriptional transactivation of Fas ligand gene expression. IUBMB Life 57, 651-658.
      • Kim, J.K., Kim, Y.K., Hong, J., Kim, S.Y., Lee, C.K., Kim, C.J., Kim, Y.S., Ahn, J.K., 2003. Isolation of the enhanced neurovirulent HSV-1 strains from Korean patients. Virus Genes 26, 115-118.
      • Kim, Y.K., Junn, E., Park, I., Lee, Y., Kang, C., Ahn, J.K., 1999. Repression of hepatitis B virus X gene expression by hammerhead ribozymes. Biochemical and Biophysical Research Communications 257, 759-765.
    [Kim, Jeong-Yoon]
    • Title : Professor International Business and Finance
    • Major : Cellular & Molecular Biotechnology
    • Phone : +82 42-821-6419
    • E-mail : jykim@cnu.ac.kr
    • Room : Building N11 – 606
    • Research Interests

      Cell Polarity Prof. Kim is interested in understanding the molecular mechanisms of cell polarity, especially the signaling pathways for the morphogenetic change during cellular differentiation and development. He studies the functions of NDR kinases, GEFs or GAPs for RhoGTPases, and scaffolding proteins affecting the formation of dendrites or spines in rat hippocampal neurons. He also investigates the roles of the NDR kinase Cbk1 and its downstream effectors in Candida albicans morphogenesis and host invasion.

      Cellular Aging Prof. Kim’s research also includes the study on as yet unknown and context-dependent functions of the sirtuin family proteins, which are a class III histone deacetylase and important in aging process. He is keen on investigating why and how the roles of sirtuins in cellular aging and stress response vary in different genetic backgrounds and environmental conditions.

    • Selected Publication
      • Moon HY, Cheon SA, Kim H, Agaphonov MO, Kwon O, Oh DB, Kim J-Y*, Kang HA*. (* co-corresponding author) 2015. Funtional and transcriptome analyses of Hansenula polymorpha Hac1p, a key UPR transcription factor, reveal a critical function in modulating protein N-glycosylation activity. Appl Environ Micriobiol. (accepted)
      • Lee HJ, Kim JM, Kang WK, Yang H, Kim J-Y. 2015. The NDR kinase Cbk1 downregulates the transcriptional repressor Nrg1 through the mRNA-binding protein Ssd1 in Candida albicans. Euk Cell. 14(7): 671-83.
      • Bal J, Lee HJ, Cheon SA, Lee KJ, Oh DB, Kim J-Y. 2013. Ylpex5 mutation partially suppresses the defective hyphal growth of a Yarrowia lipolytica ceramide synthase mutant, Yllac1, by recovering lipid raft polarization and vacuole morphogenesis. Fungal Genet Biol. 50:1-10.
      • Cheon SA, Bal J, Song Y, Hwang HM, Kim AR, Kang WK, Kang HA, Hannibal-Bach HK, Knudsen J, Ejsing CS, Kim J-Y. 2012. Distinct roles of two ceramide synthases, CaLag1p and CaLac1p, in the morphogenesis of Candida albicans. Mol Microbiol. 83(4):728-45.
      • Kim J-Y, Oh MH, Bernard LP, Macara IG, Zhang H. 2011. The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons. J Biol Chem. 286(43):37615-24.
    [Rho, Jaerang]
    • Title : Professor International Business and Finance
    • Major : Immunology & Medical Microbiology
    • Phone : +82 42-821-6420
    • E-mail : jrrho@cnu.ac.kr
    • Room : Building N11 – 612
    • Research Interests

      The term “Osteoimmunology”has been coined to encourage an interdisciplinary approach to understanding the cross-talk between bone and the immune system. Despite extensive cross-talk between bone metabolism and the immune system, the mechanisms by which one regulates the other, and the biological implications of such interactions, are poorly understood. It has recently come to be appreciated that the skeletal and immune systems regulate each other to a much greater degree than previously believed. In particular, various pathological conditions which lead to excessive bone loss, such as rheumatoid arthritis, osteoarthritis, periodontal diseases, and some tumorassociated bone abnormalities have been shown to be influenced by cellular components (e.g., Tlymphocytes) as well as by soluble factors produced by infiltrating lymphocytes (e.g., interferon produced by infiltrating lymphocytes). Future preventative treatments for these bone-related diseases that impact quality of life will require a high degree of specificity and selectivity. In this regard, my research is focused on the molecular analysis of the osteoimmune system. Better understanding of how the osteoimmune system operates in normal and pathological situations is likely to lay the groundwork for future therapies for the variety of diseases that affect both bone and the immune system. Current research projects: (1) Study how osteoclast differentiation or function is regulated (2) Study how TRAF6 modulates RANK- or TLRmediated signaling in bone or immune system (3) Study how TRAF2 controls TNF-induced inflammation (4) Study how MDSC regulates autoimmune inflammatory disease or cancer development.

    • Selected Publication
      • Park ES, Choi S, Shin B, Yu J, Yu J, Hwang JM, Yun H, Chung YH, Choi JS, Choi Y, Rho J. TRAF-interacting protein (TRIP) negatively regulates the TRAF2 ubiquitin-dependent pathway by suppressing the TRAF2-S1P interaction. Journal of Biological Chemistry. 2015. Apr 10; 290(15): 9660-9673.
      • Amarasekara DS, Yu J, Park ES, Choi S, Yu J, Rho J. Bone Loss Triggered by the Cytokine Network in Inflammatory Autoimmune Diseases. Journal of Immunology Research. 2015. May 1. 2015:832127.
      • Park JE, Park ES, Yu JE, Rho J, Paudel S, Hyun BH, Yang DK, Shin HJ. Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus. Virus Research. 2015 Feb 2;197:108-15.
      • Shin B, Yu J, Park ES, Choi S, Yu J, Hwang JM, Yun H, Chung YH, Hong KS, Choi JS, Takami M, Rho J. Secretion of a truncated Ostm1 mutant inhibits osteoclastogenesis through downregulation of the Blimp1-NFATc1 axis. Journal of Biological Chemistry. 2014. Dec 26;289(52):35868-81.
      • Kang MR, Jo SA, Yoon YD, Park KH, Oh SJ, Yun J, Lee CW, Nam KH, Kim Y, Han SB, Yu J, Rho J, Kang JS. Agelasine D Suppresses RANKL-Induced Osteoclastogenesis via Down-Regulation of c-Fos, NFATc1 and NF-κB. Marine Drugs. 2014. Nov 24;12(11):5643-56.
      • Lim H, Cheong HK, Rho JR, Hyun JK, Kim YJ. Expression, purification and characterization of human vacuolar-type H(+)-ATPase subunit d1 and d2 in Escherichia coli. Protein Expression and Purification. 2014. June 01. 98: 25-31.
      • Park ES, Kim J, Ha TU, Choi JS, Hong KS & Rho J. TDAG51 deficiency promotes oxidative stress-induced apoptosis through the generation of reactive oxygen species in mouse embryonic fibroblasts. Experimental & Molecular Medicine. 2013. 45. e35.
      • Yu J, Choi S, Park ES, Shin B, Yu J, Lee SH, Takami M, Kang JS, Meong H, Rho J. 2012. Dchiro-inositol Negatively Regulates the Formation of Multinucleated Osteoclasts by Down-Regulating NFATc1. Journal Clinical Immunology. 32(6):1360-71.
      • Barrow AD, Raynal N, Andersen TL, Slatter DA, Bihan D, Pugh N, Cella M, Kim T, Rho J, Negishi-Koga T, Delaisse JM, Takayanagi H, Lorenzo J, Colonna M, Farndale RW, Choi Y, Trowsdale J. OSCAR is a collagen receptor that costimulates osteoclastogenesis in DAP12-deficient humans and mice. Journal of Clinical Investigation. 2011. 121(9):3505-16.
      • Kim T, Ha H, Kim N, Park ES, Rho J, Kim EC, Lorenzo J, Choi Y, Lee SH. ATP6v0d2 deficiency increases bone mass, but does not influence ovariectomy-induced bone loss. Biochemical and Biophysical Research Communication. 2010. 403(1):73-8.
      • Yu J, Shin B, Park ES, Yang S, Choi S, Kang M, Rho J. Protein arginine methyltransferase 1 regulates herpes simplex virus replication through ICP27 RGGbox methylation. Biochemical and Biophysical Research Communication. 2009. 391(1):322-8.
      • Lee SH, Kim T, Park ES, Yang S, Jeong D, Choi Y, Rho J. NHE10, an osteoclastspecific member of the Na+/H+ exchanger family, regulates osteoclast differentiation and survival. Biochemical and Biophysical Research Communication. 2008. 369(2):320-6.
      • Park ES, Choi S, Kim JM, Jeong Y, Choe J, Park CS, Choi Y, Rho J. Early embryonic lethality caused by targeted disruption of the TRAF-interacting protein (TRIP) gene. Biochemical and Biophysical Research Communication. 2007. 363(4):971-7.
      • Lee S-H, J Rho, D Jeong, J-Y Sul, T Kim, N Kim, J-S Kang, T Miyamoto, T Suda, S-K Lee, R J Pignolo, B Koczon-Jaremko, J Lorenzo & Y Choi. v-ATPase V0 subunit d2-deficient mice exhibit impaired osteoclast fusion and increased bone formation. Nature Medicine. 2006. 12(12):1403-9.
      • Walsh MC, N Kim,Y Kadono, J Rho, SY Lee, J Lorenzo, and Y Choi. Osteoimmunology: Interplay Between the Immune System and Bone Metabolism. Annual Review of Immunology. 2006. 24: 33-63.
      • Hayashida, N. Inouye, M Fujimoto, Y Tanaka, H Izu, E Takaki, H Ichikawa, J Rho and A Nakai. A novel HSF1-mediated death pathway that is suppressed by heat shock proteins. EMBO J. 2006. 25(20):4773-83.
    [Kim, Seung-Bum]
    • Title : Professor International Business and Finance
    • Major : Microbial Phylogenetics & Environmental Genomics
    • Phone : +82 42-821-6412
    • E-mail : sbk01@cnu.ac.kr
    • Room : Building N11 – 608
    • Research Interests

      My research interests are focused on the ‘Diversity and Functions of Prokaryotes in Environment’, which include the following areas.

      Molecular systematics and evolution: Taxonomic characterization of prokaryotes using molecular phylogenetic methods, and description of novel taxa.

      Environmental microbiome: Analysis of prokaryotic diversity using next-generation sequencing (NGS) technology, identification of ‘core microbiome’, and elucidation of its functions using metabolic properties and transcriptomics

      Genomics: Genome analysis of prokaryotes that constitute core microbiome in environment, and also those that produce bioactive substances

    • Selected Publication
      • Han JH, Kim TS, Joung Y, Kim MN, Shin KS, Bae T, Kim, SB. 2013. Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots. Int J Syst Evol Microbiol. 63: 4730-4734.
      • Nedashkovskaya OI, Cho SH, Joung Y, Joh K, Kim MN, Shin KS, Oh HW, Bae KS, Mikhailov VV, Kim, SB. 2013. Altererythrobacter troitsensis sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol. 63: 93-97.
      • Kim KO, Shin KS, Kim MN, Shin KS, Labeda DP, Han JH, Kim, SB. 2012. Reassessment of the status of Streptomyces setonii and reclassification of Streptomyces fimicarius as a later synonym of Streptomyces setonii and Streptomyces albovinaceus as a later synonym of Streptomyces globisporus based on combined 16S rRNA/gyrB gene analysis. Int J Syst Evol Microbiol. 62: 2978-2985.
      • Kim TU, Cho SH, Han JH, Shin YM, Kim, SB. 2012. Diversity and physiological properties of root endophytic actinobacteria in native herbaceous plants of Korea. J Microbiol. 50: 50-57. Han JH, Cho MH, Kim, SB. 2012. Ribosomal and protein coding gene based multigene phylogeny on the family Streptomycetaceae. Syst Appl Microbiol. 35: 1-6.
    [Lee, Soojin]
    • E-mail :Title : Professor International Business and Finance
    • Major : Molecular Microbiology & Functional Genomics
    • Phone : +82 42-821-6414
    • E-mail : leesoojin@cnu.ac.kr
    • Room : Building N11 – 610
    • Research Interests

      New cancer drug targets through extensive DNA microassay analysis To identify new cancer drug targets, we examined global changes in gene expression between tumor biopsies and normal tissues. Several unknown genes were identified as genes exhibiting significant differential expression in multiple human cancer tissues. Among those, we identified a gene, Cancerupregulated gene 2 (CUG2), which is significantly upregulated in several human tissues such as ovary and liver. Recent our studies also revealed that CUG2 play multiple roles by interacting with various key proteins including CENP-T, an important regulator in the interaction between kinetochore and mitotic spindle during mitosis, and CSN5, a key regulator in the proteasome-mediated protein degradation. CUG2 may play important regulatory roles in cell division and mitosis by modulating the cellular activities of these interacting proteins. Our goal is to investigate the cellular function of the newly-identified cancer-related genes in tumor biogenesis and to develop potentially promising new targets for cancer therapy.

      Target validation for new anti-obesity drug development We newly identified a gene, human kilon, which displayed a commonly down-regulated expression in multiple tumor biopsies. This protein is identified as GPI-anchored protein and localized in lipid raft subcompartment of cell membrane. Recent reports revealed that the genetic variation of human kilon is highly associated with the human overweight. By using kilon knockout mice, we are investigating the cellular function of human kilon to reveal the molecular basis of the link between kilon and human obesity and to validate if kilon can be used as a future anti-obesity drug target.

    • Selected Publication
      • Chun Y, Lee M, Park B, Lee S. 2013 CSN5/JAB1 Interacts with the centromeric components CENP-T and CENP-W and regulates their proteasome-mediated degradation. J. Biol. Chem. 288 (38): 27208-19.
      • Chun Y, Park B, Koh W, Lee S, Cheon Y, Kim R, Che L, Lee S. 2011 New centromeric component CENP-W is a RNA-associated nuclear matrix protein that interacts with nucleophosmin /B23 protein J. Biol. Chem. 286(49):42758-69.
      • Jung J, Kim JM, Park B, Cheon Y, Lee B, Choo SH, Koh SS, Lee S. 2010 Newly identified tumor-associated role of human Sharpin. Mol. Cell. Biochem. 340:161-167.