• 1977-1983: Seoul National University, B.S.
• 1984-1990: University of Illinois at Chicago, Ph.D.
• 1989-1991: Yale University(HHMI), Postdoctoral fellow
• 1991-present: Biochemistry, College of Natural Sciences

Cancer therapy using membrane-bound form of cytokines

• 72. Hyun-Jin Kim, Sang Min Park, Hayyoung Lee and Young Sang Kim (2016) Membrane-bound p35 subunit of IL-12 on tumor cells is functionally equivalent to membrane-bound heterodimeric single chain IL-12 for induction of anti-tumor immunity. Immune Network 16: 305-310.
• 72. Hyeon Soo Eom, Hae Ran Park, Sung Kee Jo, Young Sang Kim, Changjong Moon, Sung-Ho Kim, Uhee Jung (2016) Ionizing Radiation Induces Altered Neuronal Differentiation by mGluR1 through PI3K-STAT3 Signaling in C17.2 Mouse Neural Stem-Like Cells. PLOS ONE 11(2):1-24.
• 73. Do Thi Van Anh, Sang Min Park, Hayyoung Lee, and Young Sang Kim (2016) The Membrane-Bound Form of IL-17A Promotes the Growth and Tumorigenicity of Colon Cancer Cells. Molecules and Cells 39: 536-542.
• 74. Tae-Young Lee, Chang-Ung Kim, Eun-Hye Bae, Sang-Hwan Seo, Dae Gwin Jeong, Sun-Woo Yoon, Kyu-Tae Chang, Young Sang Kim, Sang-Hyun Kim, Doo-Jin Kim (2017) Outer membrane vesicles harboring modified lipid A moiety augment the efficacy of an influenza vaccine exhibiting reduced endotoxicity in a mouse model. Vaccine 35: 586-595.
• 75. Van Anh Do Thi, Sang Min Park, Hayyoung Lee, Young Sang Kim (2018) Ectopically Expressed Membrane-bound Form of IL-9 Exerts Immune-stimulatory Effect on CT26 Colon Carcinoma Cells. Immune Network 18(1): 1-16.
• 76. Eun-Hye Bae, Sang Hwan Seo, Chang-Ung Kim, Min Seong Jang, Min-Suk Song Tae-Young Lee, Yu-Jin Jeong, Moo-Seung Lee, Jong-Hwan Park, Pureum Lee, Young Sang Kim, Sang-Hyun Kim, Doo-Jin Kim (2019) Bacterial Outer Membrane Vesicles Provide Broad-Spectrum Protection against Influenza Virus Infection via Recruitment and Activation of Macrophages. Journal of Innate Immunity 11(4): 316-329.
• 77. Hyun-Jung An, Mihyeun Ryub, Hye Jin Jeong, Minho Kang, Hyung-Min Jeon, Jie-Oh Lee, Young Sang Kim, Hayyoung Leee, (2019) Higd-1a regulates the proliferation of pancreatic cancer cells through a pERK/p27KIP1/pRB pathway. Cancer Letters 461: 78–89
• 78. Emerson B. Lee, Hyung-Min Jeon, Chang-Ung Kim, Sang M. Park, Geunyoung Cho, Hyun-Jin Kim, Youngjin Kim, Doo-Jin Kim, Young S. Kim, Hayyoung Lee, Jie-Oh Lee, (2019) Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage. Nanomedicine: Nanotechnology, Biology, and Medicine 17: 223–235.
• 79. Van Anh Do Thi, Hyung Min Jeon, Sang Min Park, Hayyoung Lee, Young Sang Kim (2019.12) Cell-Based IL-15:IL-15Rα Secreting Vaccine as an Effective Therapy for CT26 Colon Cancer in Mice. Molecules and Cells 42(12): 869-883.
• 80. Tae-Young Lee, Chang-Ung Kim, Pureum Lee, Sang-Hwan Seo, Eun-Hye Bae, Young Sang Kim, Sang-Hyun Kim, Doo-Jin Kim (2020.03) Outer membrane vesicle increases the efficacy of an influenza vaccine in a diet-induced obese mouse model. Immune Letter 219: 27-33
• 81. Sang Min Park, Van Anh Do-Thi, Jie-Oh Lee, Hayyoung Lee, Young Sang Kim (2020.05) Interleukin-9 Inhibits Lung Metastasis of Melanoma through Stimulating Anti-Tumor M1 Macrophages Molecules and Cells 43(5): 479-490
• 82. Van Anh Do-Thi, Jie-Oh Lee, Hayyoung Lee, Young Sang Kim (2020.11) Crosstalk between the Producers and Immune Targets of IL-9. Immune Network 20(6): e45
• 83. Van Anh Do-Thi, Hayyoung Lee, Hye Jin Jeong, Jie-Oh Lee, and Young Sang Kim (2021.08) Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System. Cancers 13:4039.
• 84. Chang-Ung Kim, Yu-Jin Jeong, Pureum Lee, Moo-Seung Lee, Jong-Hwan Park, Young-Sang Kim, Doo-Jin Kim (2022.06) Extracellular nucleoprotein exacerbates influenza virus pathogenesis by activating Toll-like receptor 4 and the NLRP3 inflammasome. Cell Mol Immunol 19(6):715-725.

• 1979-1985 Department of Chemistry, (BSc, MSc), Chungnam National University
• 1988-1993 (PhD) Department of Biochemistry, McGill University

• 1994-1996 (Postdoc) Section of Biochemistry, Molecular and Cellular Biology,Cornell University
• 1997-2003 (Assistant∼Associate Professor) Eulji Medical College

• Flavin Biochemistry: Biosynthesis of Riboflavin,Isotoplog of Riboflavin, Flavoenzyme
• Bioluminescence: Genes and Enzymes for Bacterial Bioluminescence, Quorum Sensing Regulation, Bacterial Enumeration
• Global Regulaton: Leucine-responsive Regulatory Protein

• Fluorescent Properties of the Tryptophan Containing Variants of Leucine-responsive Regulatory Proteins. J. Kor.Chem.Soc. 63, 505-510 (2019)
• Threonyl-tRNA Synthetase-mediated Translation Machinery. Nature Communication 10. 1357-1371. (2019)
• Site Directed Mutagenesis Study of LuxG as a Flavin Reductase from Bioluminescent bacteria of Photobacterium leiognathi. J.Kor.Chem. Soc. 64, 225-230 (2020)
• Evaluation of Water Solubilization Technology of Musk through Anti-inflammatory Effect Research. Kor. Soc. Biotech. Bioeng. J. 35, 244-250 (2020)
• Generation of Fluorescent Bacteria with the Genes Coding for Lumazine Protein and Riboflavin Biosynthesis. Sensors, 21, 4506.http://doi.org/10.3390/s21134506 (2021)
• Generation, Purification, and Riboflavin Synthase Variants from Bioluminescent Bacteria of Photobacterium leiognathi. Kor.J.Microbiol. 57, 154-161 (2021)
• Detoxification of the Veenom its Anti-inflammatory Activity and Decreases its Cytotoxicity and Allergenic Activity. Applied Biochemistry and Biotechnology, http://doi.org/10.1007/s12010-021-036532-2 (2021)
• Spectroscopic Analysis of Amino-Terminal Half of Riboflavin Synthase Variants Proteins. J.Kor.Chem. Soc. 65, 489-494 (2021)

• 1990.3 ~ 1994.2 B.S. Chemistry, Department of Chemistry, Seoul National University
• 1994.3 ~ 1996.2 M.S. Biochemistry, Department of Chemistry, Seoul National University
• 1996.3 ~ 2001.2 Ph.D. Biochemistry, Department of Chemistry, Seoul National University
• 2001.3 ~ 2001.4 Postdoctoral researcher, The Research Institute of Basic Sciences, Seoul National University
• 2001.4 ~ 2002.9 Postdoctoral researcher, Dept. of Biopharmaceutical and Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, USA
• 2002.9 ~ 2002.11 Postdoctoral researcher, The Research Institute of Basic Sciences, Seoul National University
• 2002.11 ~ 2003.10 Postdoctoral researcher, Brain Korea 21 Division of Chemistry and Molecular Engineering, School of Chemistry, Seoul National University
• 2003.11 ~ 2004.2 BK21 Assistant Professor, School of Chemistry, Seoul National University
• 2004.2 ~ 2006.2 Full-time Lecturer, Department of Biochemistry, College of Natural Sciences, Chungnam National University
• 2006.3 ~ 2010.2 Assistant Professor, Department of Biochemistry, College of Natural Sciences, Chungnam National University
• 2007.4 ~ 2009.2 Dept. Chair, Department of Biochemistry, College of Natural Sciences, Chungnam National University
• 2010.3 ~ 2014.8 Associate Professor, Department of Biochemistry, College of Natural Sciences, Chungnam National University
• 2014.9~ present Professor, Department of Biochemistry, College of Natural Sciences, Chungnam National University

Over the course of my research career, I have focused on aspects of gene regulatory mechanisms that are important to many fields of biological investigation. This interest began with my research in transcriptional regulatory networks in endocrine and cancer cells and grew to include research investigating epigenetic gene regulation-mediated innate immunity mechanisms in the immune system. I currently am interested in post-transcriptional gene regulation, including alternative pre-mRNA splicing (AS) in mammalian systems. I have used these different experiences frequently in my current research and I believe that the breadth of my unique experience is a valuable asset which allows me to tackle new studies from a unique perspective.

The goal of my project is to investigate the detailed mechanisms regulating AS through crosstalk mechanisms between histone modifications and its interacting RBPs. I hope to gain a comprehensive insight into this promising and unexplored field which lies at the frontier of epigenetics and RNA biology. In summary, I will address the question as to whether epigenetic mechanisms such as nucleosome positioning and histone marks play an important role in AS. Overall, the results of my research project will allow us to open a new window on the AS regulatory mechanism.

• 2003 – 2007: Ph.D., Department of Biochemistry, Chungnam National University, Daejeon, South Korea
• 2001 – 2003: M.S., Department of Biochemistry, Chungnam National University, Daejeon, South Korea
• 1994 – 2000: B.S., Department of Biochemistry, Chungnam National University, Daejeon, South Korea

• 2019 – Present: Associate Professor, Department of Biochemistry, Chungnam National University, Daejeon, Korea
• 2015 – 2019: Assistant Professor, Department of Biochemistry, Chungnam National University, Daejeon, Korea
  -Functional genomic study for human diseases using Genome- and Transcriptome-wide association studies.
• 2014 – presentable015: Principle Investigator, NIH Pathway to Independence Award, National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD, USA
  -Studying the detailed mechanisms regulating alternative splicing (AS) through crosstalk mechanisms between histone modifications and its interacting RNA-binding proteins (RBPs).
• 2012 – 2014: Research Fellow, Laboratory of Molecular Cardiology (Chief: Robert S. Adelstein) NHLBI, NIH at Bethesda, MD, USA
  -Searching tissue and/or cell specific Rbfox target genes by genome-wide analysis using next-generation sequencing. In the results, we have demonstrated that Rbfox3 controls the biogenesis of a subset of microRNAs.
• 2007 – 2012: Visiting Fellow, Laboratory of Molecular Cardiology (Chief: Robert S. Adelstein) NHLBI, NIH at Bethesda, MD, USA
  -Studying the mechanisms by which Fox proteins, a family of sequence-specific RNA binding proteins, regulate AS.

• 1. Kee K. Kim, Yanqin Yang, Jun Zhu, Robert S. Adelstein, and Sachiyo Kawamoto (2014) Rbfox3 Controls the Biogenesis of a Subset of MicroRNAs. Nat Struct Mol Biol, 21:901-910 Corresponding authors (KKK and SK)
• 2. Kee K. Kim, Robert S. Adelstein and Sachiyo Kawamoto (2014) Isoform specific proteasomal degradation of Rbfox3 during chicken embryonic development. Biochem Biophys Res Commun, 450 (4): 1662-1667
• 3. Kee K. Kim, Joseph Nam, Yoh-suke Mukouyama and Sachiyo Kawamoto (2013) Rbfox3-regulated Alternative Splicing of Numb Promotes Neuronal Differentiation in the Developing Spinal Cord. J Cell Biol. 200(4):443-458
• 4. Key Sun Park*, Kee K. Kim*, Zheng-Hao Piao, Mi Kyung Kim, Hyun Jean Lee, Yong Chan Kim, Ki Sung Lee, Jeung-Hoon Lee, Kyoon Eon Kim Olfactomedin4 suppresses tumor growth and metastasis of mouse melanoma cells through downregulation of integrin and MMP genes. Mol Cells. 34(6):555-561 *equal contribution
• 5. Key Sun Park, Kee K. Kim and Kyoon Eon Kim (2 012) Histone modification-mediated Lhx2 gene expression. Biochem Biophys Res Commun, 427(4):718-724
• 6. Kee K. Kim, Yong C. Kim, Robert S. Adelstein and Sachiyo Kawamoto (2011) Fox-3 and PSF Interact to Activate Neural Cell-specific Alternative Splicing. Nucleic Acids Res. 39:3064-3078
• 7. Kee K. Kim*, Key Sun Park*, Seok B. Song and Kyoon Eon Kim (2010). Insulin Represses Transcription of the TSHβ-subunit Gene through Increased Recruitment of NFI. J Biol Chem. 285:32003-11 *equal contribution
• 8. Yong Chan Kim, Kee K. Kim and Ethan M. Shevach (2010). Simvastatin Induces Foxp3+ T Regulatory Cells by Modulation of Transforming Growth Factor-β Signal Transduction. Immunology. 130:484-93.
• 9. Kee K. Kim, Key S. Park, Seok B. Song and Kyoon Eon Kim (2010). Up Regulation of GW112 Gene by NFkappaB Promotes an Antiapoptotic Property in Gastric Cancer Cells. Mol Carcinog. 49:259-270.
• 10. Kee K. Kim, Robert S. Adelstein and Sachiyo Kawamoto (2009). Identification of Neuronal Nuclei (NEUN) as Fox-3, a New Member of the Fox-1 Gene Family of Splicing Factors. J Biol Chem. 284:31052-61 -Cover ISSUE
• 11. Kee K. Kim, Seok B. Song, Kwang Il Kang, MyungChul Rhee and Kyoon Eon Kim (2007) Activation of the Thyroid Stimulating Hormone β-subunit Gene by LIM Homeodomain Transcription Factor Lhx2. Endocrinology. 148:3468-76.
• 12. Seok B. Song, Kee K. Kim and Kyoon Eon Kim (2002) T Vectors with Endoglucanase A (celA) Gene for Direct Detection of PCR Clones. Biotechniques. 32: 248-252.

• 2007 – 2011: Ph.D., Department of Life Science, University of Seoul, South Korea
• 2005 – 2007: M.S., Department of Life Science, University of Seoul, South Korea
• 1998 – 2005: B.S., Department of Life Science, University of Seoul, Korea

• 2017 – 2019: Researcher, Rare Disease Research Center, KRIBB, Daejeon, Korea
• 2015 – 2017: Postdoctoral fellow, Harvard School of Dental Medicine, Boston, USA
• 2012 – 2015: Postdoctoral fellow, National Human Genome Research Institute, NIH, Bethesda, USA
• 2011 – 2012: Postdoctoral fellow, Department of Life Science, University of Seoul, Seoul, Korea

• 1. Choi S, Lee HS, Cho N, Kim I, Kim EM*, Kim W*, Kim KK*. RBFOX2-regulated TEAD1 alternative splicing plays a pivotal role in Hippo-YAP signaling. Nucleic Acids Res 2022 Aug 26;50(15):8658-8673 (*Co-corresponding authors).
• 2. Choi EY, Park HH, Kim H, Kim HN, Kim I, Jeon S, Kim W*, Bae JS*, Lee W*. Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for SARS-CoV-2 patients. Eur Respir J 2020 Nov 26;56(5):2001531 (*Co-corresponding authors)
• 3. Kim J, Kwon H, Shin YK, Song G, Lee T, Kim Y, Jeong W, Lee U, Zhang X, Nam G, Jung HC, Kim W*, Jho EH*. MAML1/2 promote YAP/TAZ nuclear localization and tumorigenesis. Proc Natl Acad Sci U S A 2020 Jun 16;117(24):13529-13540. (*Co-corresponding authors)
• 4. Kim W, Cho YS, Wang X, Park O, Ma X, Kim H, Gan W, Jho EH, Cha B, Jeung YJ, Zhang L, Gao B, Wei W, Jiang J, Chung KS, Yang Y. Hippo signaling is intrinsically regulated during cell cycle progression by APC/CCdh1. Proc Natl Acad Sci U S A 2019 May 116(19): 9417-9422
• 5. Kim W*, Khan SK*, Liu Y*, Xu R, Park O, Cha B, Gao B, Yang Y. Hepatic Hippo signaling inhibits pro-tumoral microenvironment to suppress hepatocellular carcinoma. Gut 2018 Sep; 67(9): 1692-1703. (*Co-first authors)
• 6. Kim Y*, Kim W*, Song Y, Kim JR, Cho K, Moon H, Ro SW, Seo E, Ryu YM, Myung SJ, Jho EH. Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability. Proc Natl Acad Sci U S A 2017 May; 114(18): 4691-4696. (*Co-first authors)
• 7. Kim W, Khan SK, Gvozdenovic-Jeremic J, Kim Y, Dahlman J, Kim H, Park O, Ishitani T, Jho EH, Gao B, Yang Y. Hippo signaling interactions with Wnt/β-catenin and Notch signaling repress liver tumorigenesis. J Clin Invest 2017 Jan 3; 127(1): 137-152.
• 8. Moon S*, Kim W*, Kim S, Kim Y, Song Y, Bilousov O, Kim J, Lee T, Cha B, Kim M, Kim H, Katanaev VL, Jho EH. Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization. EMBO Rep 2017 Jan; 18(1): 61-71. (*Co-first authors)
• 9. Kim W*, Kim SY*, Kim T*, Kim M, Bae DJ, Choi HI, Kim IS, Jho EH. ADP-ribosylation factors 1 and 6 regulate Wnt/β-catenin signaling via control of LRP6 phosphorylation. Oncogene 2013 Jul 11;32(28): 3390-6. (*Co-first authors)
• 10. Kim W*, Kim H*, Katanaev VL, Lee SJ, Ishitani T, Cha B, Han JK, Jho EH. Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning. EMBO J 2012 Jul 6; 31(16): 3384-97. (*Co-first authors)
• 11. Cha B*, Kim W*, Kim YK, Hwang BN, Park SY, Yoon JW, Park WS, Cho JW, Bedford MT, Jho EH. Methylation by protein arginine methyltransferase 1 increases stability of Axin, a negative regulator of Wnt signaling. Oncogene 2011 May 19; 30(20): 2379-89. (*Co-first authors)

• 1998-2002: Seoul National University, B.S.
• 2004-2006: Catholic University of Korea, M.S.
• 2006-2012: Catholic University of Korea, Ph.D.

• 2012-2013: Catholic University of Korea, Postdoctoral fellow
• 2013-2019: National Institute on Aging, Postdoctoral fellow
• 2019-2020: Chonnam National University, Associate professor
• 2020-present: Chungnam National University, Associate professor

• Molecular mechanism of cell senescence/Aging and age-related disordersAnalysis of RNA-protein complexes/Mitochondrial RNA transport

• Noh JH, Kim KM, Pandey PR, Hooten NN, Munk R, Kundu G, De S, Martindale JL, Yang X, Evans MK, Abdelmohsen K, Gorospe M*. Loss of RNA-binding proteins GRSF1 activates mTOR to elicit a proinflammatory transcriptional program, Nucleic Acids Res. 2019 Mar; 47(5): 2472-2486
• Noh JH, Kim KM, Idda ML, Martindale JL, Yang X, Abdelmohsen K, and Gorospe M*. GRSF1 suppresses cell senescence, AGING. 2018 Aug; 10(8): 1856-1866.
• Kim KM, Noh JH, Bodogai M, Martindale JL, Pandey PR, Yang X, Biragyn A, Abdelmohsen K, and Gorospe M. SCAMP4 enhances the senescent cell secretome, Genes Dev. 2018 Jul; 32(13-14): 909-914.
• Noh JH, Kim KM, McClusky WG, Abdelmohsen K, Gorospe M. Cytoplasmic functions of long noncoding RNAs, Wiley Interdiscip Rev RNA. 2018 May; 9(3): e1471.
• Kim KM, Noh JH, Bodogai M, Martindale JL, Yang X, Indig FE, Basu SK, Ohnuma K, Morimoto C, Johnson PF, Biragyn A, Abdelmohsen K, and Gorospe M. Identification of senescent-cell surface targetable protein DPP4, Genes Dev. 2017 Aug; 31(15): 1529-1534.
• Noh JH, Kim KM, Abdelmohsen K, Yoon JH, Panda AC, Munk R, Curtis J, Moad CA, Indig FE, de Paula W, Dudekula DB, De S, Yang X, Martindale JL, de Cabo R, and Gorospe M. HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP, Genes Dev. 2016 May; 30(10): 1224-39.

• 2010 – 2014: Ph.D. in Molecular Cell Biology, Department of Biological Sciences, Sungkyunkwan University (Advisor: Cheol Yong Choi, Ph.D.)
• 2008 – 2010: M.S. in Molecular Cell Biology, Department of Biological Sciences, Sungkyunkwan University (Advisor: Cheol Yong Choi, Ph.D.)
• 2004 – 2008: B.S in Department of Biological Sciences, Sungkyunkwan University

• Biochemical mechanisms of fuel metabolism and the functional relevance in human physiolgy and pathology

• 2021/03 - : Assistant Professor, Department of Biochemistry, College of Natural Sciences, Chungnam National University
• 2016/04 - : Research Fellow, Division of Metabolic Diseases, Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School (Mentor: Nika N. Danial, Ph.D.)
• 2014/03 - : Postdoctoral Fellow, Lab of Molecular Genetics, Department of Biological Sciences, Sungkyunkwan University (Mentor: Cheol Yong Choi, Ph.D.)

• 1. Yoo and Choi* et al., MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation. Cell Reports, 2022 in press. Co-corresponding author
• 2. Choi* and Roh et al., Development of a novel fluorescent biosensor for dynamic monitoring of metabolic methionine redox status in cells and tissues. Biosensors and bioelectronics. 2021 Feb 15;178:113031. Co-first author
• 3. Lane and Choi* et al., HCF-1 regulates de novo lipogenesis through a nutrient sensitive complex with ChREBP. Molecular Cell. 2019 Jul 25;75(2):357-371. Co-first author
• 4. Kim and Choi* et al,. The deubuiquitinating enzyme USP20 stabilize ULK1 and promotes autophagy initiation. EMBO reports. 2018 Apr;19(4). pii: e44378. Co-first author
• 5. Choi* and Na et al,. WIP1, a Homeostatic Regulator of the DNA Damage Response, Is Targeted by HIPK2 for Phosphorylation and Degradation. Molecular Cell. 2013 July 16 8;51(3):374-85. Co-first author